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KMID : 1007520220310131741
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Food Science and Biotechnology
2022 Volume.31 No. 13 p.1741 ~ p.1751
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Protein from Hylocereus polyrhizus protects MRC-5 cells against hydrogen peroxide (H2O2)-induced damage
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Ding Haomiao
Zhang Yuzhe
Zha Yue
Zhou Sijia
Cao Chaoqing
Zhu Huajian
Xu Feng
Zhang Xiuqiang
Wang Caisheng
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Abstract
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The cytoprotective and potential molecular mechanisms of Hylocereus polyrhizus protein (RFPP) were investigated on the hydrogen peroxide (H2O2)-triggered damage in normal human embryonic lung (MRC-5) cells. An MTT assay was conducted to assess the MRC-5 cell viability after exposure to H2O2 or RFPP. Cell cycle distribution and apoptosis were explored via flow cytometry. The contents of related proteins were assessed via western blot. MRC-5 cells exhibited markedly decreased cellular viability after treatment with H2O2; however, treatment with RFPP suppressed this decrease. Additionally, RFPP interference dampened H2O2-triggered intracellular apoptosis levels and increased H2O2-triggered intracellular S phase. In these processes, the contents of phosphorylated (p)-AKT along with p-mTOR proteins were downregulated in 120 μM H2O2-treated cells compared with vehicle-treated cells. Nevertheless, in MRC-5 cells inoculated with RFPP, the levels expression of these proteins were reversed. To conclude, RFPP protected MRC-5 cells from H2O2-triggered damage via activation of the PI3K/AKT/mTOR cascade.
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KEYWORD
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Hylocereus polyrhizus, Protein, MRC-5 cells, Damage, PI3K/AKT/ mTOR cascade
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